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KMID : 0882420110800010056
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Korean Journal of Medicine
2011 Volume.80 No. 1 p.56 ~ p.62
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The Effect of Pantoprazole on Bone Turnover in Ovariectomized ICR Mice
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Lim Eun-Hye
Kim Key-Hyeon
Lee Beom-Jae
Joo Moon-Kyung
Koh Jin-Sung
Lee Joon-Young
Lim Sang-Ah
Kim Ji-Hoon
Yeon Jong-Eun
Park Jong-Jae
Kim Jae-Seon
Byun Kwan-Soo
Park Young-Tae
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Abstract
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Background/Aims: Long-term exposure to proton pump inhibitors is associated with osteoporosis-related fractures; however, the mechanism is unknown. The purpose of this study was to evaluate the effect of pantoprazole on osteoporosis and bone turnover in ovariectomized ICR mice fed a calcium-free diet.
Methods: Ovariectomized female ICR mice were divided into a pantoprazole group (n=10) and a control group (n=10). The mice in the pantoprazole group were given an intraperitoneal injection of pantoprazole at 20 mg/kg twice daily. After 4 weeks, the mice were humanely euthanized, and bone mineral density (BMD) and dry tibia weight were measured. Serum osteocalcin and CTX-1 levels were measured by enzyme-linked immunosorbent assay. The mRNA expression levels of cytokines that stimulate osteoclast differentiation were determined using RT-PCR. Serum calcium, phosphorus, and alkaline phosphatase (ALP) levels were also analyzed.
Results: Serum osteocalcin concentration was significantly lower in the pantoprazole group compared with the control group (p=0.023). There was no difference in BMD, dry tibia weight, or serum ALP, calcium, phosphorus, or CTX-1 between the two groups. The expression of interleukin (IL)-1β was lower in the pantoprazole group compared with the control group, but not significantly lower (p=0.058). The levels of tumor necrosis factor-α and IL-6 did not differ between the two groups.
Conclusions: Pantoprazole, a proton pump inhibitor, decreased serum osteocalcin and suppressed IL-1β expression, suggesting that pantoprazole affects bone formation and resorption in ovariectomized ICR mice. Further studies using larger sample sizes are needed.
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KEYWORD
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Pantoprazole, Osteoporosis, Bone turnover
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